Fish in sperms and icsi

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Typically men with unknown genetic abnormalities resort to using assisted reproductive technologies ART to achieve their reproductive goals. Infertile men who father biological children using ART could have a higher incidence of aneuploidy, which is a deviation from the normal haploid or diploid chromosomal state. Aneuploidy can be evaluated using fluorescent in situ hybridization FISH , a cytogenetic assay that gives an estimate of the frequencies of chromosomal abnormalities. The chromosomes that are generally analyzed in FISH are associated with aneuploidies that are compatible with life, that is, chr. The technique is indicated for a variety of reasons, but most importantly in the following: 1 men who despite normal semen parameters suffer recurrent pregnancy loss and 2 men with normal semen parameters, undergoing IVF, but still experiencing recurrent implantation failure. It may be used as a screening tool to help in reproductive and genetic counseling of affected couples, or those who have previously experienced failure of ART.
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Focusing on male genetic material

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"Intracytoplasmic sperm injection (ICSI) in fishes" by German Antonio Poleo

Genetic abnormalities, including sperm chromosome aneuploidy as well as structural aberrations, are one of the major causes of infertility. The use of chromosome-specific DNA probes labeled with fluorochromes, particularly the combination with multiple probes, has been used to indirectly study the sperm chromosome by fluorescent in situ hybridization FISH. Clinically, this technique is also used to assess the sperm of men recovering from gonadotoxic treatment. Recent advances in this technology facilitate the evaluation of sperm aneuploidy. Automation of FISH imaging and analysis, as well as the development of emerging techniques such as comparative genomic hybridization, will all contribute to the promise of future diagnostic approaches aimed at improving the quality, ease, and efficiency of aneuploidy analysis.
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Intracytoplasmic sperm injection ICSI is a technique that has been successfully used for assisting reproduction in mammals. However, this method is still not reliable in nonmammalian species, including teleosts. We succeeded in producing medaka individuals by ICSI with a rate of
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Intracytoplasmic sperm injection ICSI in fishes. Sperm from zebrafish, Danio rerio, and Nile tilapia, Oreochromis niloticus, were microinjected directly into egg cytoplasm to evaluate the potential for developing a novel method of fertilization. In zebrafish, the sperm of two lines wild-type and gold, long-fin were injected with or without activation into activated and non-activated eggs. Damage of maternal chromosomes by the injection procedure could have caused the developmental abnormalities observed after ICSI.
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